《植物生理学报》 2015, 51(10): 1729-1734

黄花马铃苣苔的离体培养与快速繁殖

潘梅, 黄赛, 戚华莎, 王景飞, 云勇^*

海南省农业科学院热带园艺研究所, 海口571100

通信作者：云；E-mail: yyong3819@163.com；Tel: 189075903361

摘　要：

本文对黄花马铃苣苔进行离体培养与快速繁殖技术研究。结果表明, 黄花马铃苣苔叶片外植体的最适初代诱导培养基为MS+0.5 mg•L^-1 6-BA+0.1 mg•L^-1 NAA, pH值为6.5。最适继代增殖培养基为MS+0.1 mg•L^-1 6-BA+0.1 mg•L^-1 NAA, 培养30 d的增殖系数为7.30。最适生根培养基为MS+0.5 mg•L^-1 IBA, 生根率100%。对生根组培苗进行移栽, 成活率最高达91.43%。

关键词：黄花马铃苣苔; 叶片; 离体培养

收稿：2015-05-12   修定：2015-08-25

资助：海南省科学事业费项目(kyys-2013-12)。

In Vitro Culture and Rapid Propagation of Oreocharis flavida

PAN Mei, HUANG Sai, QI Hua-Sha, WANG Jing-Fei, YUN Yong^*

Tropical Horticulture Research Institute of Hainan Academy of Agricultural Sciences, Haikou 571100, China

Corresponding author: YUN Yong; E-mail: yyong3819@163.com; Tel: 189075903361

Abstract:

We investigated in vitro culture and rapid propagation of Oreocharis flavida. The optimal medium for bud induction from leaves was MS+0.5 mg•L^-1 6-BA+0.1 mg•L^-1 NAA (pH 6.5). The optimal medium for subculture of bud multiplication was MS+0.1 mg•L^-1 6-BA+0.1 mg•L^-1 NAA, and the proliferation coefficient was 7.30 at 30 d. The optimal rooting medium was MS+NAA 0.5 mg•L^-1, the rooting rate was 100%. The rooting plantlets were transplanted in the matix and the highest survival rate was 91.43%.

Key words: Oreocharis flavida; leaf; in vitro culture